SEEING MOLECULES IN LIVING CELLS

We watch the molecular motion picture of a cell

RESEARCH

Many revolutions in biology have been followed by breakthroughs in microscopy and chemistry.  
Our research program is aimed to explore new frontiers of optical microscopy that may drive new revolutions in our understandings of life. 

SUPER-RESOLUTION FLUORESCENCE 
MICROSCOPY

The resolution limit of optical microscopy is shattered by super-resolution fluorescence microscopy, awarded 2014 Nobel Prize in Chemistry. We explore new venues of STORM (STochastic Optical Reconstruction Microscopy) including resolution down to nanometer, time window lasting days and color channels up to thousands. These advances may lay stepping stones for collecting big data of biological systems spanning multiple scales of space, time and molecules.

SUPER-RESOLUTION 
RAMAN
MICROSCOPY

In super-resolution fluorescene microscopy, there remains the intrinsic resolution limit set by fluorescent probes. We pursue ways to achieve diffraction-unlimited resolution of label-free optical microscopy such as Raman scattering. Since vibrational probes for Raman contrast is composed of a few atoms, the label-limited resolution can be vastly reduced. Our research will lay a stepping stone for achieving label-free, molecular-specific, molecular-resolution imaging tool.

IN SITU
SINGLE-CELL SYSTEMS BIOLOGY

In situ sequencing may revolutionize systems biology to access the spatial heterogeneity of gene expression. The current techniques in their infancy require new chemistry and microscopy tools for vastly improving the throughput.  For instance, our light sheet microscope may enable faster and longer reading. We also develop a new combinatorial labeling chemistry for in situ genomics and proteomics. These methods will enable us to map the comprehensive molecular landscapes of a cell.  

Label-free Super-resolution
Molecules for New Contrast
In Situ Proteomics
STORM Optics
Live-cell STORM: ER & mito
3D STORM: Neuron
Live-cell STORM: the ER
Live-cell STORM: lipid diffusion
3D STORM: clathrin
3D STORM: mitochondrial fission
 
SITEMAP
ABOUT

sangheeshim@korea.ac.kr

145 Anam-Ro, Seongbuk-Gu

Seoul 02841, Korea

02841 서울시 성북구 안암로 145, 고려대학교 R&D센터

LINKS

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Molecules for New Contrast

We study the spectroscopy of novel fluorescent probes and apply them to super-resolution optical microscopy and in situ proteomics.